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TSPAN12 Antibody

LS-C681623
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTSPAN12
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TSPAN12 Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IF, IHC, IHC-P
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23554
  • Target name
    TSPAN12
  • Target description
    tetraspanin 12
  • Target synonyms
    EVR5, NET-2, NET2, TM4SF12, tetraspanin-12, tetraspan NET-2, transmembrane 4 superfamily member 12, tspan-12
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TSPAN12 using anti-TSPAN12 antibody (A05472-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysates, Lane 2: human SW620 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSPAN12 antigen affinity purified polyclonal antibody (Catalog # A05472-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSPAN12 at approximately 43KD. The expected band size for TSPAN12 is at 35KD.
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