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Formalin-fixed and paraffin embedded mouse small intestine labeled with Anti TSPAN20/UPIb Polyclonal Antibody, PE conjugated (bs-9426R-PE) 1:200, 60 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei
Formalin-fixed and paraffin embedded mouse small intestine labeled with Anti TSPAN20/UPIb Polyclonal Antibody, PE conjugated (bs-9426R-PE) 1:200, 60 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei
Formalin-fixed and paraffin embedded mouse small intestine labeled with Anti TSPAN20/UPIb Polyclonal Antibody, PE conjugated (bs-9426R-PE) 1:200, 60 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei

TSPAN20 Polyclonal Antibody

BS-9426R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetUPK1B
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Overview

  • Supplier
    Bioss
  • Product Name
    TSPAN20 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-2000), ELISA(ELISA=1:5000-10000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7348
  • Target name
    UPK1B
  • Target description
    uroplakin 1B
  • Target synonyms
    TSPAN20, UPIB, UPK1, uroplakin-1b, UP1b, tetraspan, tetraspanin-20, tspan-20, uroplakin Ib
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75841
  • Protein Name
    Uroplakin-1b
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of Uroplakin Ib/UPIb using anti-Uroplakin Ib/UPIb antibody (A10230-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: rat stomach tissue lysates, Lane 4: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Uroplakin Ib/UPIb antigen affinity purified polyclonal antibody (Catalog # A10230-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Uroplakin Ib/UPIb at approximately 30KD. The expected band size for Uroplakin Ib/UPIb is at 30KD.
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