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IHC-P analysis of mouse embryo tissue using GTX51813 TSPYL5 antibody. Dilution : 1:200
IHC-P analysis of mouse embryo tissue using GTX51813 TSPYL5 antibody. Dilution : 1:200
IHC-P analysis of mouse embryo tissue using GTX51813 TSPYL5 antibody. Dilution : 1:200

TSPYL5 antibody

GTX51813
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetTSPYL5
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Overview

  • Supplier
    GeneTex
  • Product Name
    TSPYL5 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:50-400. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID85453
  • Target name
    TSPYL5
  • Target description
    TSPY like 5
  • Target synonyms
    testis-specific Y-encoded-like protein 5; TSPY-like protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86VY4
  • Protein Name
    Testis-specific Y-encoded-like protein 5
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TSPYL5 using anti-TSPYL5 antibody (A10648-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSPYL5 antigen affinity purified polyclonal antibody (Catalog # A10648-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSPYL5 at approximately 48 kDa. The expected band size for TSPYL5 is at 45 kDa.
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