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Western Blot Positive WB detected in: Rat liver tissue All lanes: TST antibody at 5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa
Western Blot Positive WB detected in: Rat liver tissue All lanes: TST antibody at 5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa
Western Blot Positive WB detected in: Rat liver tissue All lanes: TST antibody at 5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa

TST Antibody

CSB-PA620980LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTST
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Overview

  • Supplier
    Cusabio
  • Product Name
    TST Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7263
  • Target name
    TST
  • Target description
    thiosulfate sulfurtransferase
  • Target synonyms
    epididymis secretory sperm binding protein; RDS; thiosulfate sulfurtransferase; thiosulfate sulfurtransferase (rhodanese)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16762
  • Protein Name
    Thiosulfate sulfurtransferase
  • Scientific Description
    Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity (By similarity). Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TST using anti-TST antibody (A00965-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TST antigen affinity purified polyclonal antibody (Catalog # A00965-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TST at approximately 34 kDa. The expected band size for TST is at 33 kDa.
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