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TTC34 Antibody (aa529-558)

LS-C203710
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetTTC34
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TTC34 Antibody (aa529-558)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID100287898
  • Target name
    TTC34
  • Target description
    tetratricopeptide repeat domain 34
  • Target synonyms
    tetratricopeptide repeat protein 34, TPR repeat protein 34, TPR repeat-containing protein ENSP00000383873
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TTC34 using anti-TTC34 antibody (A19531-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat testis tissue lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC34 antigen affinity purified polyclonal antibody (A19531-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTC34 at approximately 61 kDa. The expected band size for TTC34 is at 61 kDa.
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