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Western blot All lanes: TTC38 antibody at 1.5microg/ml Lane 1: U251 whole cell lysate Lane 2: Mouse liver tissue Lane 3: HepG2 whole cell lysate Lane 4: K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa
Western blot All lanes: TTC38 antibody at 1.5microg/ml Lane 1: U251 whole cell lysate Lane 2: Mouse liver tissue Lane 3: HepG2 whole cell lysate Lane 4: K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa
Western blot All lanes: TTC38 antibody at 1.5microg/ml Lane 1: U251 whole cell lysate Lane 2: Mouse liver tissue Lane 3: HepG2 whole cell lysate Lane 4: K562 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53 kDa Observed band size: 53 kDa

TTC38 Antibody

CSB-PA719094LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTTC38
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Overview

  • Supplier
    Cusabio
  • Product Name
    TTC38 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55020
  • Target name
    TTC38
  • Target description
    tetratricopeptide repeat domain 38
  • Target synonyms
    LL22NC03-5H6.5; tetratricopeptide repeat protein 38; TPR repeat protein 38
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5R3I4
  • Protein Name
    Tetratricopeptide repeat protein 38
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TTC38 using anti-TTC38 antibody (A16794-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC38 antigen affinity purified polyclonal antibody (Catalog # A16794-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTC38 at approximately 53 kDa. The expected band size for TTC38 is at 53 kDa.
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