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Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate All lanes: TTC39B antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 77, 67, 76, 60, 70, 23 kDa Observed band size: 77 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate All lanes: TTC39B antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 77, 67, 76, 60, 70, 23 kDa Observed band size: 77 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate All lanes: TTC39B antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 77, 67, 76, 60, 70, 23 kDa Observed band size: 77 kDa

TTC39B Antibody

CSB-PA713348LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTTC39B
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Overview

  • Supplier
    Cusabio
  • Product Name
    TTC39B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID158219
  • Target name
    TTC39B
  • Target description
    tetratricopeptide repeat domain 39B
  • Target synonyms
    C9orf52, tetratricopeptide repeat protein 39B, TPR repeat protein 39B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5VTQ0
  • Protein Name
    Tetratricopeptide repeat protein 39B
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TTC39B using anti-TTC39B antibody (A13852-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTC39B antigen affinity purified polyclonal antibody (Catalog # A13852-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTC39B at approximately 70 kDa. The expected band size for TTC39B is at 70 kDa.
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