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Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Rat kidney tissue All lanes: TTI2 antibody at 8.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Rat kidney tissue All lanes: TTI2 antibody at 8.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Rat kidney tissue All lanes: TTI2 antibody at 8.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57 kDa Observed band size: 57 kDa

TTI2 Antibody

CSB-PA764687LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetTTI2
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Overview

  • Supplier
    Cusabio
  • Product Name
    TTI2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80185
  • Target name
    TTI2
  • Target description
    TELO2 interacting protein 2
  • Target synonyms
    C8orf41, MRT39, TELO2-interacting protein 2, Tel2 interacting protein 2 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6NXR4
  • Protein Name
    TELO2-interacting protein 2
  • Scientific Description
    Regulator of the DNA damage response (DDR). Part of the TTT complex that is required to stabilize protein levels of the phosphatidylinositol 3-kinase-related protein kinase (PIKK) family proteins. The TTT complex is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), ultraviolet (UV) and mitomycin C (MMC). Together with the TTT complex and HSP90 may participate in the proper folding of newly synthesized PIKKs.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TTI2 using anti-TTI2 antibody (A12943-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTI2 antigen affinity purified polyclonal antibody (Catalog # A12943-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTI2 at approximately 60 kDa. The expected band size for TTI2 is at 57 kDa.
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