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Immunohistochemistry of paraffin-embedded human lymph node tissue using CSB-PA025251LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lymph node tissue using CSB-PA025251LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lymph node tissue using CSB-PA025251LA01HU at dilution of 1:100

TTK Antibody

CSB-PA025251LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTTK
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Overview

  • Supplier
    Cusabio
  • Product Name
    TTK Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7272
  • Target name
    TTK
  • Target description
    TTK protein kinase
  • Target synonyms
    cancer/testis antigen 96; CT96; dual specificity protein kinase TTK; ESK; monopolar spindle 1 kinase; monopolar spindle 1-like 1; MPH1; MPS1; MPS1L1; phosphotyrosine picked threonine kinase; phosphotyrosine picked threonine-protein kinase; PYT
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP33981
  • Protein Name
    Dual specificity protein kinase TTK
  • Scientific Description
    Phosphorylates proteins on serine, threonine, and tyrosine. Probably associated with cell proliferation. Essential for chromosome alignment by enhancing AURKB activity (via direct CDCA8 phosphorylation) at the centromere, and for the mitotic checkpoint.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TTK using anti-TTK antibody (A01132-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat testicular tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTK antigen affinity purified polyclonal antibody (Catalog # A01132-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTK at approximately 97KD. The expected band size for TTK is at 97KD.
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