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IHC-P analysis of colon tissue using GTX83473 TTLL12 antibody [4B2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
IHC-P analysis of colon tissue using GTX83473 TTLL12 antibody [4B2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50
IHC-P analysis of colon tissue using GTX83473 TTLL12 antibody [4B2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min. Dilution : 1:50

TTLL12 antibody [4B2]

GTX83473
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetTTLL12
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Overview

  • Supplier
    GeneTex
  • Product Name
    TTLL12 antibody [4B2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. ICC/IF: 1:100. IHC-P: 1:50. FCM: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4B2
  • Concentration
    0.73 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23170
  • Target name
    TTLL12
  • Target description
    tubulin tyrosine ligase like 12
  • Target synonyms
    dJ526I14.2, tubulin--tyrosine ligase-like protein 12, inactive tubulin--tyrosine ligase-like protein 12, tubulin tyrosine ligase-like family, member 12
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ14166
  • Protein Name
    Tubulin--tyrosine ligase-like protein 12
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TTLL12 using anti-TTLL12 antibody (A14589-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTLL12 antigen affinity purified polyclonal antibody (Catalog # A14589-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTLL12 at approximately 75 kDa. The expected band size for TTLL12 is at 74 kDa.
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