More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/TTLL12 Mouse Monoclonal Antibody [Clone ID: OTI4B2]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TTLL12 (Cat# RC200841, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTLL12(Cat# TA500789). Positive lysates [LY402417] (100ug) and [LC402417] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TTLL12 (Cat# RC200841, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTLL12(Cat# TA500789). Positive lysates [LY402417] (100ug) and [LC402417] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TTLL12 (Cat# RC200841, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTLL12(Cat# TA500789). Positive lysates [LY402417] (100ug) and [LC402417] (20ug) can be purchased separately from OriGene.

TTLL12 Mouse Monoclonal Antibody [Clone ID: OTI4B2]

CF500789
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTTLL12
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    TTLL12 Mouse Monoclonal Antibody [Clone ID: OTI4B2]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4B2
  • Gene ID23170
  • Target name
    TTLL12
  • Target description
    tubulin tyrosine ligase like 12
  • Target synonyms
    dJ526I14.2, tubulin--tyrosine ligase-like protein 12, inactive tubulin--tyrosine ligase-like protein 12, tubulin tyrosine ligase-like family, member 12
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ14166
  • Protein Name
    Tubulin--tyrosine ligase-like protein 12
  • Scientific Description
    Carrier-free (BSA/glycerol-free) TTLL12 mouse monoclonal antibody, clone OTI4B2 (formerly 4B2)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Figure 1. Western blot analysis of TTLL12 using anti-TTLL12 antibody (A14589-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTLL12 antigen affinity purified polyclonal antibody (Catalog # A14589-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTLL12 at approximately 75 kDa. The expected band size for TTLL12 is at 74 kDa.
Product group Antibodies
Boster Bio
Anti-TTLL12 Antibody Picoband(r)A14589-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetTTLL12
  • SizePrice
Anti-TTLL12 Antibody
Product group Antibodies
Antibodies.com
Anti-TTLL12 AntibodyA306568
ApplicationsWestern Blot
ReactivityHuman
  • SizePrice
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Product group Antibodies
Atlas Antibodies
Anti-TTLL12 AntibodyHPA003054
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetTTLL12
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
KIAA0153 / TTLL12 AntibodyLS-C409733
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetTTLL12
  • SizePrice
TTLL12 antibody detects TTLL12 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TTLL12 antibody (GTX120613) diluted by 1:3000.
Product group Antibodies
GeneTex
TTLL12 antibody [C1C3]GTX120613
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetTTLL12
  • SizePrice