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ProductsBack/TTLL12 Mouse Monoclonal Antibody [Clone ID: OTI6E4]
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells(WT) and TTLL12-Knockdown HeLa cells(KD) were separated by SDS-PAGE and immunoblotted with anti-TTLL12 monoclonal antibody TA500816(1:2500).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells(WT) and TTLL12-Knockdown HeLa cells(KD) were separated by SDS-PAGE and immunoblotted with anti-TTLL12 monoclonal antibody TA500816(1:2500).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells(WT) and TTLL12-Knockdown HeLa cells(KD) were separated by SDS-PAGE and immunoblotted with anti-TTLL12 monoclonal antibody TA500816(1:2500).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.

TTLL12 Mouse Monoclonal Antibody [Clone ID: OTI6E4]

CF500816
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTTLL12
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    TTLL12 Mouse Monoclonal Antibody [Clone ID: OTI6E4]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI6E4
  • Gene ID23170
  • Target name
    TTLL12
  • Target description
    tubulin tyrosine ligase like 12
  • Target synonyms
    dJ526I14.2, tubulin--tyrosine ligase-like protein 12, inactive tubulin--tyrosine ligase-like protein 12, tubulin tyrosine ligase-like family, member 12
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ14166
  • Protein Name
    Tubulin--tyrosine ligase-like protein 12
  • Scientific Description
    Carrier-free (BSA/glycerol-free) TTLL12 mouse monoclonal antibody, clone OTI6E4 (formerly 6E4)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TTLL12 using anti-TTLL12 antibody (A14589-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTLL12 antigen affinity purified polyclonal antibody (Catalog # A14589-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTLL12 at approximately 75 kDa. The expected band size for TTLL12 is at 74 kDa.
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