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ProductsBack/TTLL2 Antibody (FITC)

TTLL2 Antibody (FITC)

LS-C397234
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetTTLL2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TTLL2 Antibody (FITC)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ELISA
  • Applications Supplier
    ELISA The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    FITC
  • Estimated Purity
    ...
  • Gene ID83887
  • Target name
    TTLL2
  • Target description
    tubulin tyrosine ligase like 2
  • Target synonyms
    C6orf104, NYD-TSPG, dJ366N23.3, probable tubulin polyglutamylase TTLL2, testis-specific protein NYD-TSPG, tubulin tyrosine ligase-like family, member 2, tubulin--tyrosine ligase-like protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TTLL2 using anti-TTLL2 antibody (A15891). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TTLL2 antigen affinity purified polyclonal antibody (Catalog # A15891) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TTLL2 at approximately 70 kDa. The expected band size for TTLL2 is at 67 kDa.
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