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Western Blot analysis of K562 cells using GCP3 Polyclonal Antibody
Western Blot analysis of K562 cells using GCP3 Polyclonal Antibody
Western Blot analysis of K562 cells using GCP3 Polyclonal Antibody

TUBGCP3 Antibody

CSB-PA002660
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTUBGCP3
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Overview

  • Supplier
    Cusabio
  • Product Name
    TUBGCP3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10426
  • Target name
    TUBGCP3
  • Target description
    tubulin gamma complex component 3
  • Target synonyms
    104p, ALP6, GCP3, Grip104, SPBC98, Spc98, Spc98p, gamma-tubulin complex component 3, gamma-ring complex protein 104 kDa, spindle pole body protein Spc98 homolog, tubulin gamma complex associated protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96CW5
  • Protein Name
    Gamma-tubulin complex component 3
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TUBGCP3 using anti-TUBGCP3 antibody (A10799-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBGCP3 antigen affinity purified polyclonal antibody (Catalog # A10799-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TUBGCP3 at approximately 104 kDa. The expected band size for TUBGCP3 is at 104 kDa.
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