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TWF2 / PTK9L Antibody

LS-C404630
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTWF2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TWF2 / PTK9L Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:25 - 1:100), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11344
  • Target name
    TWF2
  • Target description
    twinfilin actin binding protein 2
  • Target synonyms
    A6r; A6-related protein; A6RP; MSTP011; protein tyrosine kinase 9-like (A6-related protein); PTK9L; PTK9L protein tyrosine kinase 9-like (A6-related protein); twinfilin, actin-binding protein, homolog 2; twinfilin-1-like protein; twinfilin-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TWF2 using anti-TWF2 antibody (A09748-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWF2 antigen affinity purified polyclonal antibody (Catalog # A09748-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TWF2 at approximately 40 kDa. The expected band size for TWF2 is at 40 kDa.
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