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Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A549 cells, 293T cells, Primary antibody: CSB-PA234430(TXLNA Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A549 cells, 293T cells, Primary antibody: CSB-PA234430(TXLNA Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A549 cells, 293T cells, Primary antibody: CSB-PA234430(TXLNA Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds

TXLNA Antibody

CSB-PA234430
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetTXLNA
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Overview

  • Supplier
    Cusabio
  • Product Name
    TXLNA Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID200081
  • Target name
    TXLNA
  • Target description
    taxilin alpha
  • Target synonyms
    IL-14, IL14, TXLN, alpha-taxilin, interleukin 14
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP40222
  • Protein Name
    Alpha-taxilin
  • Scientific Description
    alpha-taxilin is a novel binding partner of the syntaxin family which is implicated in intracellular vesicle trafficking. Through its C-terminal coiled-coil region, alpha-taxilin interacts with the nascent polypeptide-associated complex (NAC), which acts as a transcriptional coactivator.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Alpha Taxilin/TXLNA using anti-Alpha Taxilin/TXLNA antibody (A05668-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Taxilin/TXLNA antigen affinity purified polyclonal antibody (Catalog # A05668-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Taxilin/TXLNA at approximately 75 kDa. The expected band size for Alpha Taxilin/TXLNA is at 62 kDa.
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