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Western Blot analysis of HL-60 Raji K562 SW480 A549 22RV-1 cells using TRP14 Polyclonal Antibody
Western Blot analysis of HL-60 Raji K562 SW480 A549 22RV-1 cells using TRP14 Polyclonal Antibody
Western Blot analysis of HL-60 Raji K562 SW480 A549 22RV-1 cells using TRP14 Polyclonal Antibody

TXNDC17 Antibody

CSB-PA004860
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTXNDC17
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Overview

  • Supplier
    Cusabio
  • Product Name
    TXNDC17 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84817
  • Target name
    TXNDC17
  • Target description
    thioredoxin domain containing 17
  • Target synonyms
    TRP14, TXNL5, thioredoxin domain-containing protein 17, 14 kDa thioredoxin-related protein, protein 42-9-9, testicular tissue protein Li 214, thioredoxin (Trx)-related protein, 14 kDa, thioredoxin-like 5, thioredoxin-like protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BRA2
  • Protein Name
    Thioredoxin domain-containing protein 17
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TXNDC17 using anti-TXNDC17 antibody (A09772-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNDC17 antigen affinity purified polyclonal antibody (Catalog # A09772-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TXNDC17 at approximately 14 kDa. The expected band size for TXNDC17 is at 14 kDa.
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WB analysis of HL-60, Raji, K562, SW480, A549, and 22RV-1 cell lysates using GTX34287 TRP14 antibody.
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