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Western Blot analysis of COS7 cells using TYR Polyclonal Antibody
Western Blot analysis of COS7 cells using TYR Polyclonal Antibody
Western Blot analysis of COS7 cells using TYR Polyclonal Antibody

TYR Antibody

CSB-PA004353
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetTYR
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Overview

  • Supplier
    Cusabio
  • Product Name
    TYR Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7299
  • Target name
    TYR
  • Target description
    tyrosinase
  • Target synonyms
    ATN; CMM8; LB24-AB; monophenol monooxygenase; OCA1; OCA1A; OCAIA; oculocutaneous albinism IA; SHEP3; SK29-AB; tumor rejection antigen AB; tyrosinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14679
  • Protein Name
    Tyrosinase
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Lane 1: Mouse Eye lysates; Lane 2: Mouse Skin lysates probed with Tyrosinase Polyclonal Antibody, Unconjugated (bs-0819R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of Tyrosinase/TYR using anti-Tyrosinase/TYR antibody (A00326-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tyrosinase/TYR antigen affinity purified polyclonal antibody (Catalog # A00326-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tyrosinase/TYR at approximately 60-65 kDa. The expected band size for Tyrosinase/TYR is at 60 kDa.
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