Tyramide Amplification Kit with HRP Goat Anti-Rabbit and CF(r)488A Tyramide

Biotium

Tyramide Amplification Kit with HRP Goat anti-Rabbit and CF 488A Tyramide

9

Labeling/Conjugation

Research Use Only

Other Conjugate

Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure. CF® dyes are Biotiums line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes. Green fluorescent CF®488A has excitation/emission at 490/515 nm. Kit Components: CF®488A tyramide stock solution HRP Goat anti-rabbit IgG BSA for blocking Amplification buffer Hydrogen peroxide 30% stablized solution We also offer Tyramide Amplification Kits with HRP goat anti-rabbit or HRP streptavidin.

Animal, Plant - IgG from domesticated goat serum (Capra aegagrus hircus); BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells; peroxidase from Horseradish (Armoracia rusticana)

-20°C

41116124