Tyramide Amplification Kit with HRP Streptavidin and CF(r)543 Tyramide

Biotium

Tyramide Amplification Kit with HRP Streptavidin and CF 543 Tyramide (Kit)

9

Labeling/Conjugation

Research Use Only

Other Conjugate

Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure. CF® dyes are Biotiums line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes. Orange fluorescent CF®543 has excitation/emission at 541/560 nm. Kit Components: CF®543 tyramide stock solution HRP Streptavidin BSA for blocking Amplification buffer Hydrogen peroxide 30% stablized solution We also offer Tyramide Amplification Kits with HRP goat anti-rabbit or HRP streptavidin.

Animal, Plant - BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells; peroxidase from Horseradish (Armoracia rusticana); recombinant streptadin from E. coli

-20°C

41116124