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Tyrosinase Antibody (Azide-free)

LS-C18404
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTYR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Tyrosinase Antibody (Azide-free)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Immunocytology, Immunofluorescence, Western Blot and Immunohistology. Western Blot: 1-2 ug/ml for 2 hours at RT. Immunohistology: 2-4 ug/ml for 30 minutes at RT (formalin/paraffin). Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Melanoma cell lines (SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37), melanocytes in skin and Melanomas (1). The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.. ICC, IF, IHC, IHC-P, WB (1 - 2 µg/ml) Suitable for use in Immunocytology, Immunofluorescence, Western Blot and Immunohistology. Western Blot: 1-2 ug/ml for 2 hours at RT. Immunohistology: 2-4 ug/ml for 30 minutes at RT (formalin/paraffin). Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Positive control: Melanoma cell lines (SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37), melanocytes in skin and Melanomas (1). The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7299
  • Target name
    TYR
  • Target description
    tyrosinase
  • Target synonyms
    ATN; CMM8; LB24-AB; monophenol monooxygenase; OCA1; OCA1A; OCAIA; oculocutaneous albinism IA; SHEP3; SK29-AB; tumor rejection antigen AB; tyrosinase
  • Host
    Mouse
  • Isotype
    IgG2a
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Tyrosinase/TYR using anti-Tyrosinase/TYR antibody (A00326-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tyrosinase/TYR antigen affinity purified polyclonal antibody (Catalog # A00326-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tyrosinase/TYR at approximately 60-65 kDa. The expected band size for Tyrosinase/TYR is at 60 kDa.
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