Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [HL2721] (GTX639548) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Tyrosine Hydroxylase antibody [HL2721] (GTX639548) diluted at 1:1000 and competitor's antibody () diluted at 1:. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639548/GTX639548_T-45257_20240112_WB_TPM_watermark_24011618_311.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Tyrosine Hydroxylase antibody [HL2721] (GTX639548) diluted at 1:1000 and competitor's antibody () diluted at 1:. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Tyrosine Hydroxylase antibody [HL2721] detects Tyrosine Hydroxylase protein at cytoplasm by immunohistochemical analysis. Sample: Frozen-sectioned mouse brain. Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [HL2721] (GTX639548) diluted at 1:100. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX639548/GTX639548_T-45257_20231229_IHC-Fr_M_24021917_831.webp "Tyrosine Hydroxylase antibody [HL2721] detects Tyrosine Hydroxylase protein at cytoplasm by immunohistochemical analysis. Sample: Frozen-sectioned mouse brain. Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [HL2721] (GTX639548) diluted at 1:100. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [HL2721] (GTX639548) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639548/GTX639548_45341_20240315_WB_R_H_24031823_890.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [HL2721] (GTX639548) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Tyrosine Hydroxylase antibody [HL2721] detects Tyrosine Hydroxylase protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse tissues. Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [HL2721] (GTX639548) diluted at 1:250. Red: GFAP antibody [GT25] (GTX641104) diluted at 1:250. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639548/GTX639548_45341_20250620_IHC-Fr_M_Multiple_RPKM_2_25062520_747.webp "Tyrosine Hydroxylase antibody [HL2721] detects Tyrosine Hydroxylase protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse tissues. Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [HL2721] (GTX639548) diluted at 1:250. Red: GFAP antibody [GT25] (GTX641104) diluted at 1:250. Blue: Hoechst 33342 staining.")
![Tyrosine Hydroxylase antibody [HL2721] detects Tyrosine Hydroxylase protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse tissues. Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [HL2721] (GTX639548) diluted at 1:250. Red: GFAP antibody [GT25] (GTX641104) diluted at 1:250. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639548/GTX639548_45341_20250620_IHC-Fr_M_Multiple_RPKM_1_25062520_104.webp "Tyrosine Hydroxylase antibody [HL2721] detects Tyrosine Hydroxylase protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse tissues. Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody [HL2721] (GTX639548) diluted at 1:250. Red: GFAP antibody [GT25] (GTX641104) diluted at 1:250. Blue: Hoechst 33342 staining.")
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody [HL2721] (GTX639548) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Tyrosine Hydroxylase antibody [HL2721]
GTX639548
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTH
Overview
- SupplierGeneTex
- Product NameTyrosine Hydroxylase antibody [HL2721]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2721
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7054
- Target nameTH
- Target descriptiontyrosine hydroxylase
- Target synonymsDYT14, DYT5b, TYH, tyrosine 3-monooxygenase, dystonia 14, tyrosine 3-hydroxylase
- HostRabbit
- IsotypeIgG
- Protein IDP07101
- Protein NameTyrosine 3-monooxygenase
- Scientific DescriptionThe protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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![IHC-P analysis of human midbrain using GTX01922 Tyrosine Hydroxylase antibody [1B5]. Note cytoplasmic staining of catecholaminergic cells and their processes.](https://www.genetex.com/upload/website/prouct_img/normal/GTX01922/GTX01922_20200811_IHC-P_103_w_23053121_765.webp)
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