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Western Blot analysis of 293 cells using Uba5 Polyclonal Antibody
Western Blot analysis of 293 cells using Uba5 Polyclonal Antibody
Western Blot analysis of 293 cells using Uba5 Polyclonal Antibody

UBA5 Antibody

CSB-PA004356
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetUBA5
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Overview

  • Supplier
    Cusabio
  • Product Name
    UBA5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79876
  • Target name
    UBA5
  • Target description
    ubiquitin like modifier activating enzyme 5
  • Target synonyms
    DEE44, EIEE44, SCAR24, THIFP1, UBE1DC1, ubiquitin-like modifier-activating enzyme 5, UBA5, ubiquitin-activating enzyme E1 homolog, UFM1-activating enzyme, ubiquitin-activating enzyme 5, ubiquitin-activating enzyme E1 domain-containing protein 1, ubiquitin-activating enzyme E1-domain containing 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9GZZ9
  • Protein Name
    Ubiquitin-like modifier-activating enzyme 5
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of UBA5 using anti-UBA5 antibody (A06385-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat pancreas tissue lysates, Lane 5: mouse pamcreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBA5 antigen affinity purified polyclonal antibody (Catalog # A06385-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBA5 at approximately 50 kDa. The expected band size for UBA5 is at 45 kDa.
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