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Western Blot analysis of Mouse pancreas tissue, Hela cell and Mouse liver tissue, A549 and 293T cell using UBB Polyclonal Antibody at dilution of 1:500
Western Blot analysis of Mouse pancreas tissue, Hela cell and Mouse liver tissue, A549 and 293T cell using UBB Polyclonal Antibody at dilution of 1:500
Western Blot analysis of Mouse pancreas tissue, Hela cell and Mouse liver tissue, A549 and 293T cell using UBB Polyclonal Antibody at dilution of 1:500

UBB Polyclonal Antibody

E-AB-10015
Elabscience
ApplicationsWestern Blot
Product group Antibodies
TargetUBB
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Overview

  • Supplier
    Elabscience
  • Product Name
    UBB Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7314
  • Target name
    UBB
  • Target description
    ubiquitin B
  • Target synonyms
    HEL-S-50, polyubiquitin-B, epididymis secretory protein Li 50, polyubiquitin B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP0CG47
  • Protein Name
    Polyubiquitin-B
  • Scientific Description
    This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimers disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Ubiquitin using anti-Ubiquitin antibody (PB9122). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ubiquitin antigen affinity purified polyclonal antibody (Catalog # PB9122) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ubiquitin at approximately 9KD. The expected band size for Ubiquitin is at 9KD.
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