Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with UBE2W Polyclonal Antibody, Unconjugated (bs-8385R ) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with UBE2W Polyclonal Antibody, Unconjugated (bs-8385R ) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
UBE2W Polyclonal Antibody
BS-8385R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetUBE2W
Overview
- SupplierBioss
- Product NameUBE2W Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID55284
- Target nameUBE2W
- Target descriptionubiquitin conjugating enzyme E2 W
- Target synonymsUBC-16, UBC16, ubiquitin-conjugating enzyme E2 W, E2 ubiquitin-conjugating enzyme W, N-terminal E2 ubiquitin-conjugating enzyme, N-terminus-conjugating E2, ubiquitin carrier protein W, ubiquitin conjugating enzyme E2 W (putative), ubiquitin conjugating enzyme E2W (putative), ubiquitin-conjugating enzyme 16, ubiquitin-conjugating enzyme 2W, ubiquitin-protein ligase W
- HostRabbit
- IsotypeIgG
- ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
- Storage Instruction-20°C
- UNSPSC12352203
Datasheet
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