Ubiquitin Antibody

LifeSpan BioSciences

Ubiquitin Antibody

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The detection of ubiquitinated species is further complicated by the fact that the process of ubiquitination is reversible and is accomplished by highly specific proteases present in all eukaryotic cells. It is therefore recommended that cell lysates are kept on ice and the lysis buffers contain one or more of cysteine protease inhibitors (n-ethylmaleimide, E64, etc.). Applications: Suitable for use in ELISA and Western Blot. ELISA: 1:1000-1:5000. Western Blot: 1:200-1:1000.. ELISA (1:1000 - 1:5000), WB (1:200 - 1:1000) The detection of ubiquitinated species is further complicated by the fact that the process of ubiquitination is reversible and is accomplished by highly specific proteases present in all eukaryotic cells. It is therefore recommended that cell lysates are kept on ice and the lysis buffers contain one or more of cysteine protease inhibitors (n-ethylmaleimide, E64, etc.). Applications: Suitable for use in ELISA and Western Blot. ELISA: 1:1000-1:5000. Western Blot: 1:200-1:1000.

Western Blot, ELISA

Research Use Only

Polyclonal

Unconjugated

Rabbit

IgG

41116161