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Ubiquitin Antibody

LS-C76659
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Ubiquitin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Western Blot. Western Blot Analysis: 0.5-2.0 ug/ml of this lot detected full-length ubiquitin from bovine erythrocytes, and ubiquitinated proteins in acid extracts from HeLa cells subjected to high temperature antigen retrieval.. WB (0.5 - 2 µg/ml) Suitable for use in Western Blot. Western Blot Analysis: 0.5-2.0 ug/ml of this lot detected full-length ubiquitin from bovine erythrocytes, and ubiquitinated proteins in acid extracts from HeLa cells subjected to high temperature antigen retrieval.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Ubiquitin using anti-Ubiquitin antibody (PB9122). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ubiquitin antigen affinity purified polyclonal antibody (Catalog # PB9122) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ubiquitin at approximately 9KD. The expected band size for Ubiquitin is at 9KD.
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