Ubiquitin Antibody

Biorbyt

Ubiquitin Antibody

16

The stated application concentrations are suggested starting points. Titration of the Ubiquitin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT IHC-P, WB

Research Use Only

Monoclonal

PBQN-1

Unconjugated

RPS27A

ribosomal protein S27a

CEP80, HEL112, S27A, UBA80, UBC, UBCEP1, UBCEP80, eS31, ubiquitin-ribosomal protein eS31 fusion protein, 40S ribosomal protein S27a, epididymis luminal protein 112, ubiquitin C, ubiquitin and ribosomal protein S27a, ubiquitin carboxyl extension protein 80, ubiquitin-40S ribosomal protein S27a, ubiquitin-CEP80

Mouse

IgG1

Polyubiquitin-B

Ubiquitin is a small (8.5 kDa) regulatory protein found in most tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or less frequently ubiquitylation. Ubiquitination affects proteins in many ways: it can mark them for degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Ubiquitination involves three main steps: activation, conjugation, and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s), respectively. The result of this sequential cascade is to bind ubiquitin to lysine residues on the protein substrate via an isopeptide bond, cysteine residues through a thioester bond, serine and threonine residues through an ester bond, or the amino group of the proteins N-terminus via a peptide bond. [Wiki]

Human

-20°C,2°C to 8°C

12352203