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The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA257638(UCHL3 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA257638(UCHL3 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA257638(UCHL3 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: x200)

UCHL3 Antibody

CSB-PA257638
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetUCHL3
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Overview

  • Supplier
    Cusabio
  • Product Name
    UCHL3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7347
  • Target name
    UCHL3
  • Target description
    ubiquitin C-terminal hydrolase L3
  • Target synonyms
    UCH-L3, ubiquitin carboxyl-terminal hydrolase isozyme L3, testicular tissue protein Li 221, ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase), ubiquitin thioesterase L3, ubiquitin thiolesterase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15374
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase isozyme L3
  • Scientific Description
    The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of UCHL3 using anti-UCHL3 antibody (M05004-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UCHL3 antigen affinity purified monoclonal antibody (Catalog # M05004-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UCHL3 at approximately 26 kDa. The expected band size for UCHL3 is at 26 kDa.
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