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Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: A431 cell, Primary antibody: CSB-PA227642(UGT1A10 Antibody) at dilution 1/250 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: A431 cell, Primary antibody: CSB-PA227642(UGT1A10 Antibody) at dilution 1/250 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: A431 cell, Primary antibody: CSB-PA227642(UGT1A10 Antibody) at dilution 1/250 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds

UGT1A10 Antibody

CSB-PA227642
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetUGT1A10
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Overview

  • Supplier
    Cusabio
  • Product Name
    UGT1A10 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54575
  • Target name
    UGT1A10
  • Target description
    UDP glucuronosyltransferase family 1 member A10
  • Target synonyms
    Bilirubin-specific UDPGT isozyme 1; GNT1; hUG-BR1; UDP glucuronosyltransferase 1 family, polypeptide A10; UDP glycosyltransferase 1 family, polypeptide A10; UDP-glucuronosyltransferase 1-1; UDP-glucuronosyltransferase 1-10; UDP-glucuronosyltransferase 1-A; UDP-glucuronosyltransferase 1A1; UDP-glucuronosyltransferase 1A10; UDP-glucuronosyltransferase 1-J; UDPGT; UGT1; UGT1.1; UGT1.10; UGT1-01; UGT1-10; UGT1A; UGT-1A; UGT1A1; UGT1J; UGT-1J
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HAW8
  • Protein Name
    UDP-glucuronosyltransferase 1-10
  • Scientific Description
    This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of UGT1A10 using anti-UGT1A10 antibody (A04459). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UGT1A10 antigen affinity purified polyclonal antibody (Catalog # A04459) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UGT1A10 at approximately 60 kDa. The expected band size for UGT1A10 is at 60 kDa.
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