More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/ULK1 Antibody
Immunofluorescent analysis of PC-3 cells using CSB-PA025612LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of PC-3 cells using CSB-PA025612LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of PC-3 cells using CSB-PA025612LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ULK1 Antibody

CSB-PA025612LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
TargetULK1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Cusabio
  • Product Name
    ULK1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8408
  • Target name
    ULK1
  • Target description
    unc-51 like autophagy activating kinase 1
  • Target synonyms
    ATG1; ATG1 autophagy related 1 homolog; ATG1A; autophagy-related protein 1 homolog; hATG1; serine/threonine-protein kinase ULK1; UNC51; Unc51.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75385
  • Protein Name
    Serine/threonine-protein kinase ULK1
  • Scientific Description
    Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Assay Genie
ULK1/ULK2 AntibodyCAB18685
TargetULK1
  • SizePrice
Anti-ULK1 Antibody
Product group Antibodies
Antibodies.com
Anti-ULK1 AntibodyA10448
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • SizePrice
Product group Antibodies
RayBiotech
Anti-ULk1 Antibody144-66638
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetULK1
  • SizePrice
Immunofluorescent staining of human cell line A549 shows localization to cytosol.
Product group Antibodies
Atlas Antibodies
Anti-ULK1 AntibodyHPA063990
ApplicationsImmunoCytoChemistry
TargetULK1
  • SizePrice
Product group Antibodies
MedChem Express
ULK1 AntibodyHY-P80366
TargetULK1
  • SizePrice
Product group Antibodies
GeneTex
ULK1 (phospho Ser758) antibodyGTX132654
ApplicationsWestern Blot
TargetULK1
  • SizePrice
293T cell lysates probed with ATG1/ULK1 Polyclonal Antibody, Unconjugated (bs-3602R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
References
Bioss Antibodies
ATG1/ULK1 Polyclonal AntibodyBS-3602R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetULK1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ULK1 Antibody (aa400-499)LS-C410064
ApplicationsImmunoFluorescence, Western Blot
TargetULK1
  • SizePrice
Figure 1. Western blot analysis of ULK1 using anti-ULK1 antibody (A00584-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ULK1 antigen affinity purified polyclonal antibody (Catalog # A00584-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ULK1 at approximately 120KD. The expected band size for ULK1 is at 120KD.
Product group Antibodies
Boster Bio
Anti-ULK1 Antibody Picoband(r)A00584-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetULK1
  • SizePrice
Western Blot of the lysates of A431 cells nontreated (-) or treated (+) with Calyculin A/Okadaic Acid, using Anti-Phospho-ULK1 (Ser757) rabbit monoclonal antibody (clone RM488) at a 1:1000 dilution.
Product group Antibodies
RevMAb Biosciences
anti-Phospho-ULK1 (Ser757), Rabbit Monoclonal (RM488)REV-31-1380-00
ApplicationsWestern Blot
TargetULK1
  • SizePrice