ULK2 antibody [C2C3], C-term detects ULK2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. ULK2 stained by ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111476/GTX111476_44258_20221216_WB_shRNA_watermark_22122018_879.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111476/GTX111476_44258_20210326_WB_w_23060500_257.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![ULK2 antibody [C2C3], C-term detects ULK2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111476/GTX111476_41703_20150529_WB_w_23060500_681.webp "ULK2 antibody [C2C3], C-term detects ULK2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![ULK2 antibody [C2C3], C-term detects ULK2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ULK2 stained by ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111476/GTX111476_41703_20190116_ICC_IF_w_23060500_694.webp "ULK2 antibody [C2C3], C-term detects ULK2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ULK2 stained by ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 μm.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111476/GTX111476_41703_20190201_WB_M_w_23060500_644.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111476/GTX111476_44727_20240830_WB_TPM_watermark_24090423_576.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
ULK2 antibody [C2C3], C-term detects ULK2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. ULK2 stained by ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ULK2 antibody [C2C3], C-term
GTX111476
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetULK2
Overview
- SupplierGeneTex
- Product NameULK2 antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.24 mg/ml
- ConjugateUnconjugated
- Gene ID9706
- Target nameULK2
- Target descriptionunc-51 like autophagy activating kinase 2
- Target synonymsATG1B, Unc51.2, serine/threonine-protein kinase ULK2
- HostRabbit
- IsotypeIgG
- Protein IDQ8IYT8
- Protein NameSerine/threonine-protein kinase ULK2
- Scientific DescriptionThis gene encodes a protein that is similar to a serine/threonine kinase in C. elegans which is involved in axonal elongation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Umar S, Palasiewicz K, Volin MV, et al. Metabolic regulation of RA macrophages is distinct from RA fibroblasts and blockade of glycolysis alleviates inflammatory phenotype in both cell types. Cell Mol Life Sci. 2021,78(23):7693-7707. doi: 10.1007/s00018-021-03978-5Read this paper
- Mohamud Y, Shi J, Tang H, et al. Coxsackievirus infection induces a non-canonical autophagy independent of the ULK and PI3K complexes. Sci Rep. 2020,10(1):19068. doi: 10.1038/s41598-020-76227-7Read this paper
- Nozawa T, Sano S, Minowa-Nozawa A, et al. TBC1D9 regulates TBK1 activation through Ca(2+) signaling in selective autophagy. Nat Commun. 2020,11(1):770. doi: 10.1038/s41467-020-14533-4Read this paper
- Cheng H, Yang ZT, Bai YQ, et al. Overexpression of Ulk2 inhibits proliferation and enhances chemosensitivity to cisplatin in non-small cell lung cancer. Oncol Lett. 2019,17(1):79-86. doi: 10.3892/ol.2018.9604Read this paper
- Heo JM, Ordureau A, Swarup S, et al. RAB7A phosphorylation by TBK1 promotes mitophagy via the PINK-PARKIN pathway. Sci Adv. 2018,4(11):eaav0443. doi: 10.1126/sciadv.aav0443Read this paper
Datasheet
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