Paraformaldehyde-fixed, paraffin embedded mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with UNC5B/UNC5H2 Polyclonal Antibody, Unconjugated (bs-11492R) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin at[1:500] for 40 minutes and DAPI staining of the nuclei.
Paraformaldehyde-fixed, paraffin embedded mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with UNC5B/UNC5H2 Polyclonal Antibody, Unconjugated (bs-11492R) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin at[1:500] for 40 minutes and DAPI staining of the nuclei.
UNC5B Polyclonal Antibody
BS-11492R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Equine, Human, Mouse, Porcine, Rabbit, Rat
Overview
- SupplierBioss
- Product NameUNC5B Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityCanine, Equine, Human, Mouse, Porcine, Rabbit, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
- Ko CL, Lin JA, Chen KY, et al. Netrin-1 Dampens Hypobaric Hypoxia-Induced Lung Injury in Mice. High Alt Med Biol. 2019,20(3):293-302. doi: 10.1089/ham.2018.0116Read this paper