![Paraformaldehyde-fixed, paraffin embedded mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with UNC5B/UNC5H2 Polyclonal Antibody, Unconjugated (bs-11492R) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin at[1:500] for 40 minutes and DAPI staining of the nuclei. Paraformaldehyde-fixed, paraffin embedded mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with UNC5B/UNC5H2 Polyclonal Antibody, Unconjugated (bs-11492R) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin at[1:500] for 40 minutes and DAPI staining of the nuclei.](https://biossantibodies.com/image-raw/12905.jpeg)
Paraformaldehyde-fixed, paraffin embedded mouse stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with UNC5B/UNC5H2 Polyclonal Antibody, Unconjugated (bs-11492R) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin at[1:500] for 40 minutes and DAPI staining of the nuclei.
UNC5B/UNC5H2 Polyclonal Antibody
![Research Use Only](static/images/certificates/ruo.jpg)
BS-11492R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Equine, Human, Mouse, Porcine, Rabbit, Rat
Overview
- SupplierBioss Antibodies
- Product NameUNC5B/UNC5H2 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200), ICC(1:100-500)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- ReactivityCanine, Equine, Human, Mouse, Porcine, Rabbit, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
- Netrin-1 Dampens Hypobaric Hypoxia-Induced Lung Injury in Mice. Ko CL et al., 2019 Sep, High Alt Med BiolRead more