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Lane 1: PC-3M Cells; Probed with UPF1 (9A3) Monoclonal Antibody (bsm-54402R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: PC-3M Cells; Probed with UPF1 (9A3) Monoclonal Antibody (bsm-54402R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: PC-3M Cells; Probed with UPF1 (9A3) Monoclonal Antibody (bsm-54402R) at 1:1000, overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

hUPF1 Recombinant Antibody

BSM-54402R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetUPF1
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Overview

  • Supplier
    Bioss
  • Product Name
    hUPF1 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IHC-P(1:200-400), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200), IP(1-2ug)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID5976
  • Target name
    UPF1
  • Target description
    UPF1 RNA helicase and ATPase
  • Target synonyms
    HUPF1, NORF1, RENT1, UTF, pNORF1, smg-2, regulator of nonsense transcripts 1, ATP-dependent helicase RENT1, UPF1 regulator of nonsense transcripts homolog, delta helicase, nonsense mRNA reducing factor 1, smg-2 homolog, nonsense mediated mRNA decay factor, up-frameshift mutation 1 homolog, up-frameshift suppressor 1 homolog, yeast Upf1p homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92900
  • Protein Name
    Regulator of nonsense transcripts 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RENT1/hUPF1 using anti-RENT1/hUPF1 antibody (PB9842). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: rat RH35 whole cell lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RENT1/hUPF1 antigen affinity purified polyclonal antibody (Catalog # PB9842) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RENT1/hUPF1 at approximately 140 kDa. The expected band size for RENT1/hUPF1 is at 124 kDa.
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