Immunohistochemical analysis of paraffin-embedded N87 xenograft, using USP15(GTX104644) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
![Wild-type (WT) and USP15 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with USP15 antibody [C3], C-term (GTX104644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104644/GTX104644_43145_20181123_WB_KO_watermark_w_23060120_614.webp "Wild-type (WT) and USP15 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with USP15 antibody [C3], C-term (GTX104644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![USP15 antibody [C3], C-term detects USP15 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: USP15 stained by USP15 antibody [C3], C-term (GTX104644) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104644/GTX104644_43040_20171220_ICC_IF_w_23060120_163.webp "USP15 antibody [C3], C-term detects USP15 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: USP15 stained by USP15 antibody [C3], C-term (GTX104644) diluted at 1:500. Blue: Hoechst 33342 staining.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with USP15 antibody [C3], C-term (GTX104644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX104644/GTX104644_43145_20180323_WB_w_23060120_266.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with USP15 antibody [C3], C-term (GTX104644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Immunohistochemical analysis of paraffin-embedded N87 xenograft, using USP15(GTX104644) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
USP15 antibody [C3], C-term
GTX104644
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetUSP15
Overview
- SupplierGeneTex
- Product NameUSP15 antibody [C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.3 mg/ml
- ConjugateUnconjugated
- Gene ID9958
- Target nameUSP15
- Target descriptionubiquitin specific peptidase 15
- Target synonymsUNPH-2, UNPH4, ubiquitin carboxyl-terminal hydrolase 15, deubiquitinating enzyme 15, ubiquitin thioesterase 15, ubiquitin thiolesterase 15, ubiquitin-specific-processing protease 15
- HostRabbit
- IsotypeIgG
- Protein IDQ9Y4E8
- Protein NameUbiquitin carboxyl-terminal hydrolase 15
- Scientific DescriptionUbiquitin (MIM 191339), a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains. The disassembly process is mediated by ubiquitin-specific proteases (USPs). Also see USP1 (MIM 603478).[supplied by OMIM]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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