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ProductsBack/USP22 antibody [N3C3]
Immunoprecipitation of USP22 protein from A549 whole cell extracts using 5 μg of USP22 antibody [N3C3] (GTX120048). Western blot analysis was performed using USP22 antibody [N3C3] (GTX120048) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunoprecipitation of USP22 protein from A549 whole cell extracts using 5 μg of USP22 antibody [N3C3] (GTX120048). Western blot analysis was performed using USP22 antibody [N3C3] (GTX120048) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunoprecipitation of USP22 protein from A549 whole cell extracts using 5 μg of USP22 antibody [N3C3] (GTX120048). Western blot analysis was performed using USP22 antibody [N3C3] (GTX120048) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

USP22 antibody [N3C3]

GTX120048
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetUSP22
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Overview

  • Supplier
    GeneTex
  • Product Name
    USP22 antibody [N3C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23326
  • Target name
    USP22
  • Target description
    ubiquitin specific peptidase 22
  • Target synonyms
    USP3L, ubiquitin carboxyl-terminal hydrolase 22, deubiquitinating enzyme 22, ubiquitin specific protease 22, ubiquitin thioesterase 22, ubiquitin thiolesterase 22, ubiquitin-specific processing protease 22
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UPT9
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase 22
  • Scientific Description
    Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Required for nuclear receptor-mediated transactivation and cell cycle progression.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of USP22 using anti-USP22 antibody (A03887-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: human MOLT-4 whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP22 antigen affinity purified polyclonal antibody (Catalog # A03887-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP22 at approximately 68 kDa. The expected band size for USP22 is at 60 kDa.
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