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Western blot All lanes: USP26 antibody at 5.22microg/ml Lane 1: Hela whole cell lysate Lane 2: U937 whole cell lysate Lane 3: Mouse gonadal tissue Lane 4: Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 105 kDa Observed band size: 105 kDa
Western blot All lanes: USP26 antibody at 5.22microg/ml Lane 1: Hela whole cell lysate Lane 2: U937 whole cell lysate Lane 3: Mouse gonadal tissue Lane 4: Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 105 kDa Observed band size: 105 kDa
Western blot All lanes: USP26 antibody at 5.22microg/ml Lane 1: Hela whole cell lysate Lane 2: U937 whole cell lysate Lane 3: Mouse gonadal tissue Lane 4: Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 105 kDa Observed band size: 105 kDa

USP26 Antibody

CSB-PA874835ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetUSP26
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Overview

  • Supplier
    Cusabio
  • Product Name
    USP26 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID83844
  • Target name
    USP26
  • Target description
    ubiquitin specific peptidase 26
  • Target synonyms
    SPGFX6, ubiquitin carboxyl-terminal hydrolase 26, deubiquitinating enzyme 26, ubiquitin specific protease 26, ubiquitin thioesterase 26, ubiquitin thiolesterase 26, ubiquitin-specific processing protease 26
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BXU7
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase 26
  • Scientific Description
    Involved in the ubiquitin-dependent proteolytic pathway in conjunction with the 26S proteasome (By similarity). Deubiquitinates the androgen receptor and regulates the androgen receptor signaling pathway.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of USP26 using anti-USP26 antibody (A07732-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP26 antigen affinity purified polyclonal antibody (Catalog # A07732-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP26 at approximately 104 kDa. The expected band size for USP26 is at 104 kDa.
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USP26 antibody [C2C3], C-term detects USP26 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: USP26 protein stained by USP26 antibody [C2C3], C-term (GTX118119) diluted at 1:500. Blue: Hoechst 33342 staining.
Product group Antibodies
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USP26 antibody [C2C3], C-termGTX118119
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