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Western Blot analysis of 293T cells using USP44 Polyclonal Antibody
Western Blot analysis of 293T cells using USP44 Polyclonal Antibody
Western Blot analysis of 293T cells using USP44 Polyclonal Antibody

USP44 Antibody

CSB-PA060300
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetUSP44
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Overview

  • Supplier
    Cusabio
  • Product Name
    USP44 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84101
  • Target name
    USP44
  • Target description
    ubiquitin specific peptidase 44
  • Target synonyms
    deubiquitinating enzyme 44; ubiquitin carboxyl-terminal hydrolase 44; ubiquitin specific protease 44; ubiquitin thioesterase 44; ubiquitin thiolesterase 44; ubiquitin-specific-processing protease 44
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H0E7
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase 44
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of USP44 using anti-USP44 antibody (A08401-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP44 antigen affinity purified polyclonal antibody (Catalog # A08401-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP44 at approximately 81KD. The expected band size for USP44 is at 81KD.
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