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IHC-P analysis of rat lung tissue using GTX57228 Uteroglobin antibody. Antigen retrieval : Boiling in sodium citrate buffer (pH6.0) for 15 mins. Dilution : 1:400
IHC-P analysis of rat lung tissue using GTX57228 Uteroglobin antibody. Antigen retrieval : Boiling in sodium citrate buffer (pH6.0) for 15 mins. Dilution : 1:400
IHC-P analysis of rat lung tissue using GTX57228 Uteroglobin antibody. Antigen retrieval : Boiling in sodium citrate buffer (pH6.0) for 15 mins. Dilution : 1:400

Uteroglobin antibody

GTX57228
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse, Rat
TargetScgb1a1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Uteroglobin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:200-1:400. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID22287
  • Target name
    Scgb1a1
  • Target description
    secretoglobin, family 1A, member 1
  • Target synonyms
    CC10, CC16, CCSP, PCB-BP, UG, UGB, Utg, uteroglobin, Blastokinin, CCPBP, PCB-binding protein, club cell 17 kDa protein, club cell phospholipid-binding protein, club cell secretory protein, club cells 10 kDa secretory protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ06318
  • Protein Name
    Uteroglobin
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of Uteroglobin using anti-Uteroglobin antibody (A02201-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse lung tissue lysate, Lane 2: mouse lung tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Uteroglobin antigen affinity purified polyclonal antibody (Catalog # A02201-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Uteroglobin at approximately 10KD. The expected band size for Uteroglobin is at 10KD.
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