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Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP8 antibody (GTX132181) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP8 antibody (GTX132181) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP8 antibody (GTX132181) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

VAMP8 antibody

GTX132181
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetVAMP8
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Overview

  • Supplier
    GeneTex
  • Product Name
    VAMP8 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.37 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8673
  • Target name
    VAMP8
  • Target description
    vesicle associated membrane protein 8
  • Target synonyms
    EDB, VAMP-8, vesicle-associated membrane protein 8, vesicle-associated membrane protein 8 (endobrevin)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BV40
  • Protein Name
    Vesicle-associated membrane protein 8
  • Scientific Description
    This gene encodes an integral membrane protein that belongs to the synaptobrevin/vesicle-associated membrane protein subfamily of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). The encoded protein is involved in the fusion of synaptic vesicles with the presynaptic membrane.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Wu SY, Wu HT, Wang YC, et al. Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo. Autophagy. 2023,19(4):1239-1257. doi: 10.1080/15548627.2022.2123098
    Read this paper

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Figure 1. Western blot analysis of VAMP8 using anti-VAMP8 antibody (M02338). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VAMP8 antigen affinity purified monoclonal antibody (Catalog # M02338) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VAMP8 at approximately 11 kDa. The expected band size for VAMP8 is at 11 kDa.
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