HEK293 cells were transfected with either emply expression vector (pSR ) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 u g lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 l/300 g lysate). DPH or SHD alpha Vav2 antiserum (2.5 l/300 g lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 l/300 g lysate). DPH or SHD anti Vav2 antisera (2.5 l/300 g lysate). Werstern blot Ab - SHD anti Vav2 antibody 1:10000.
HEK293 cells were transfected with either emply expression vector (pSR ) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 u g lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 l/300 g lysate). DPH or SHD alpha Vav2 antiserum (2.5 l/300 g lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 l/300 g lysate). DPH or SHD anti Vav2 antisera (2.5 l/300 g lysate). Werstern blot Ab - SHD anti Vav2 antibody 1:10000.
VAV2 antibody
GTX16136
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetVAV2
Overview
- SupplierGeneTex
- Product NameVAV2 antibody
- Delivery Days Customer9
- Application Supplier NoteIP: Use at an assay dependent dilution. WB: Use at a dilution of 1/5000. Predicted molecular weight: 101 kDa. Optimal dilutions/concentrations should be determined by the end user.
- ApplicationsImmunoPrecipitation, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID7410
- Target nameVAV2
- Target descriptionvav guanine nucleotide exchange factor 2
- Target synonymsguanine nucleotide exchange factor VAV2; vav 2 oncogene; VAV-2
- HostSheep
- IsotypeIgG
- Protein IDP52735
- Protein NameGuanine nucleotide exchange factor VAV2
- Scientific DescriptionVAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203