VA(Vitamin A) ELISA Kit

Biorbyt

VA(Vitamin A) ELISA Kit

10

standard: 1000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of VA in the samples is then determined by comparing the OD of the samples to the standard curve

15.63-1000 ng/mL

9.06 ng/mL

Research Use Only

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vitamin AKit(VA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vitamin AKit(VA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Vitamin AKit(VA) in the samples is then determined by comparing the OD of the samples to the standard curve.

All Species

41116158