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ProductsBack/VCAM1 / CD106 antibody [M/K-2.7]

VCAM1 / CD106 antibody [M/K-2.7]

GTX14360
GeneTex
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, Neutralisation/Blocking
Product group Antibodies
ReactivityMouse
TargetVcam1
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Overview

  • Supplier
    GeneTex
  • Product Name
    VCAM1 / CD106 antibody [M/K-2.7]
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    M/K-2.7
  • Conjugate
    Unconjugated
  • Gene ID22329
  • Target name
    Vcam1
  • Target description
    vascular cell adhesion molecule 1
  • Target synonyms
    CD106, Vcam-1, vascular cell adhesion protein 1, V-CAM 1
  • Host
    Rat
  • Isotype
    IgG1
  • Protein IDP29533
  • Protein Name
    Vascular cell adhesion protein 1
  • Reactivity
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

References

  • Liver sinusoidal endothelial cell expressed vascular cell adhesion molecule 1 promotes liver fibrosis.
    Read this paper
  • VCAM-1 Density and Tumor Perfusion Predict T-cell Infiltration and Treatment Response in Preclinical Models. Riegler J et al., 2019 Oct, Neoplasia
    Read this paper

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Figure 1. Western blot analysis of VCAM1 using anti-VCAM1 antibody (A01199). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: rat spleen tissue lysates, Lane 4: rat lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCAM1 antigen affinity purified polyclonal antibody (Catalog # A01199) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCAM1 at approximately 110KD. The expected band size for VCAM1 is at 81KD.
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