More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/VCL / Vinculin Antibody (clone VIN-54)

VCL / Vinculin Antibody (clone VIN-54)

LS-C171297
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetVCL
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    VCL / Vinculin Antibody (clone VIN-54)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    IHC, IHC-Fr, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    VIN-54
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7414
  • Target name
    VCL
  • Target description
    vinculin
  • Target synonyms
    CMD1W; CMH15; epididymis luminal protein 114; epididymis secretory sperm binding protein; HEL114; metavinculin; meta-vinculin; MV; MVCL; vinculin; vinculin isoform meta-VCL
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Assay Genie
LDB1 AntibodyCAB17580
TargetVCL
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Vinculin Polyclonal Antibody, Unconjugated (bs-6640R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Product group Antibodies
References
Bioss Antibodies
Vinculin Polyclonal AntibodyBS-6640R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetVCL
  • SizePrice
Product group Antibodies
Biomatik
VCL Polyclonal AntibodyCAC14137
ApplicationsWestern Blot, ELISA
TargetVCL
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-Vinculin Antibody, Affinity PurifiedA302-534A
ApplicationsWestern Blot
TargetVCL
  • SizePrice
Anti-VCL Antibody
Product group Antibodies
Antibodies.com
Anti-VCL AntibodyA39079
ApplicationsWestern Blot, ImmunoHistoChemistry
  • SizePrice
Figure 1. Western blot analysis of VCL using anti-VCL antibody (A01207-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human SK-OV-3 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: human K562 whole cell lysates, Lane 8: human SGC-7901 whole cell lysates, Lane 9: rat heart tissue lysates, Lane 10: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCL antigen affinity purified polyclonal antibody (Catalog # A01207-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCL at approximately 124KD. The expected band size for VCL is at 124KD.
Product group Antibodies
Boster Bio
Anti-Vinculin/VCL Antibody Picoband(r)A01207-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetVCL
  • SizePrice
Product group Antibodies
RayBiotech
Anti-VCL Antibody144-01758
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetVCL
  • SizePrice
Product group Antibodies
Biosynth
Vinculin antibody10C-CR1199M1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
VCL / Vinculin AntibodyLS-C832548
ApplicationsWestern Blot, ELISA
TargetVCL
  • SizePrice
Immunohistochemistry analysis in human smooth muscle and cerebral cortex tissues using HPA002131 antibody. Corresponding VCL RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-VCL AntibodyHPA002131
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetVCL
  • SizePrice