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Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Vinculin Polyclonal Antibody, Unconjugated (bs-6640R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Vinculin Polyclonal Antibody, Unconjugated (bs-6640R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Vinculin Polyclonal Antibody, Unconjugated (bs-6640R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Vinculin Polyclonal Antibody

BS-6640R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetVCL
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Overview

  • Supplier
    Bioss
  • Product Name
    Vinculin Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7414
  • Target name
    VCL
  • Target description
    vinculin
  • Target synonyms
    CMD1W, CMH15, HEL114, MV, MVCL, VINC, vinculin, epididymis luminal protein 114, epididymis secretory sperm binding protein, meta-vinculin, metavinculin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18206
  • Protein Name
    Vinculin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Yunes-Medina L, Paciorkowski A, Nuzbrokh Y, et al. Depletion of transglutaminase 2 in neurons alters expression of extracellular matrix and signal transduction genes and compromises cell viability. Mol Cell Neurosci. 2018,86:72-80. doi: 10.1016/j.mcn.2017.11.011
    Read this paper
  • Dhawan U, Pan HA, Shie MJ, et al. The Spatiotemporal Control of Osteoblast Cell Growth, Behavior, and Function Dictated by Nanostructured Stainless Steel Artificial Microenvironments. Nanoscale Res Lett. 2017,12(1):86. doi: 10.1186/s11671-016-1810-1
    Read this paper
  • Dhawan U, Pan HA, Lee CH, et al. Spatial Control of Cell-Nanosurface Interactions by Tantalum Oxide Nanodots for Improved Implant Geometry. PLoS One. 2016,11(6):e0158425. doi: 10.1371/journal.pone.0158425
    Read this paper
  • He J, Meng G, Yao R, et al. The essential role of inorganic substrate in the migration and osteoblastic differentiation of mesenchymal stem cells. J Mech Behav Biomed Mater. 2016,59:353-365. doi: 10.1016/j.jmbbm.2016.02.013
    Read this paper
  • Jiang YH, Yang CH, Li W, et al. Aqueous extracts of Tribulus terrestris protects against oxidized low-density lipoprotein-induced endothelial dysfunction. Chin J Integr Med. 2016,22(3):193-200. doi: 10.1007/s11655-015-2321-0
    Read this paper
  • Jiang YH, Sun W, Li W, et al. Calycosin-7-O-β-D-glucoside promotes oxidative stress-induced cytoskeleton reorganization through integrin-linked kinase signaling pathway in vascular endothelial cells. BMC Complement Altern Med. 2015,15:315. doi: 10.1186/s12906-015-0839-5
    Read this paper
  • Li W, Sun W, Yang CH, et al. Tanshinone II a protects against lipopolysaccharides-induced endothelial cell injury via Rho/Rho kinase pathway. Chin J Integr Med. 2014,20(3):216-23. doi: 10.1007/s11655-013-1380-3
    Read this paper

Datasheet

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Figure 1. Western blot analysis of VCL using anti-VCL antibody (A01207-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human SK-OV-3 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: human K562 whole cell lysates, Lane 8: human SGC-7901 whole cell lysates, Lane 9: rat heart tissue lysates, Lane 10: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCL antigen affinity purified polyclonal antibody (Catalog # A01207-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCL at approximately 124KD. The expected band size for VCL is at 124KD.
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Immunohistochemistry analysis in human smooth muscle and cerebral cortex tissues using HPA002131 antibody. Corresponding VCL RNA-seq data are presented for the same tissues.
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