IHC-P analysis of human breast adenocarcinoma tissue using GTX83426 VEGFA antibody [4E3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
![IHC-P analysis of human breast tissue using GTX83426 VEGFA antibody [4E3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83426/GTX83426_1359_IHC-P_w_23061419_637.webp "IHC-P analysis of human breast tissue using GTX83426 VEGFA antibody [4E3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human liver tissue using GTX83426 VEGFA antibody [4E3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83426/GTX83426_1360_IHC-P_w_23061419_325.webp "IHC-P analysis of human liver tissue using GTX83426 VEGFA antibody [4E3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human pancreas tissue using GTX83426 VEGFA antibody [4E3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83426/GTX83426_1361_IHC-P_w_23061419_792.webp "IHC-P analysis of human pancreas tissue using GTX83426 VEGFA antibody [4E3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![WB analysis of various cell lines using GTX83426 VEGFA antibody [4E3]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX83426/GTX83426_3611_WB_w_23061419_772.webp "WB analysis of various cell lines using GTX83426 VEGFA antibody [4E3]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of HEK293T cells transfected with VEGFA plasmid (Right) or empty vector (Left) for 48 hrs using GTX83426 VEGFA antibody [4E3]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83426/GTX83426_3619_WB_w_23061419_386.webp "WB analysis of HEK293T cells transfected with VEGFA plasmid (Right) or empty vector (Left) for 48 hrs using GTX83426 VEGFA antibody [4E3]. Loading : 5 ug per lane")
![ICC/IF analysis of COS7 cells transiently transfected with VEGFA plasmid using GTX83426 VEGFA antibody [4E3].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83426/GTX83426_666_ICCIF_w_23061419_578.webp "ICC/IF analysis of COS7 cells transiently transfected with VEGFA plasmid using GTX83426 VEGFA antibody [4E3].")
IHC-P analysis of human breast adenocarcinoma tissue using GTX83426 VEGFA antibody [4E3]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
VEGFA antibody [4E3]
GTX83426
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetVEGFA
Overview
- SupplierGeneTex
- Product NameVEGF antibody [4E3] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. ICC/IF: 1:100. IHC-P: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7422
- Target nameVEGFA
- Target descriptionvascular endothelial growth factor A
- Target synonymsMVCD1; vascular endothelial growth factor A; vascular endothelial growth factor A121; vascular endothelial growth factor A165; vascular permeability factor; VEGF; VPF
- HostMouse
- IsotypeIgG1
- Protein IDP15692
- Protein NameVascular endothelial growth factor A
- Scientific DescriptionGrowth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Tissue-Material Integration and Biostimulation Study of Collagen Acellular Matrices. Quintero Sierra LA et al., 2022 Jun, Tissue Eng Regen MedRead more
- Immunolocalization of leptin and leptin receptor in colorectal mucosa of ulcerative colitis, Crohns disease and control subjects with no inflammatory bowel disease. Merigo F et al., 2021 Mar, Cell Tissue ResRead more
- Artificial dermal substitutes for tissue regeneration: comparison of the clinical outcomes and histological findings of two templates. De Francesco F et al., 2020 Aug, J Int Med ResRead more