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FACS analysis of HUVEC cells using GTX17567 VEGF Receptor 3 antibody [818CT12.1.1]. Green : Primary antibody Blue : Isotype control Dilution : 1:25
FACS analysis of HUVEC cells using GTX17567 VEGF Receptor 3 antibody [818CT12.1.1]. Green : Primary antibody Blue : Isotype control Dilution : 1:25
FACS analysis of HUVEC cells using GTX17567 VEGF Receptor 3 antibody [818CT12.1.1]. Green : Primary antibody Blue : Isotype control Dilution : 1:25

VEGF Receptor 3 antibody [818CT12.1.1]

GTX17567
GeneTex
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetFLT4
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Overview

  • Supplier
    GeneTex
  • Product Name
    VEGF Receptor 3 antibody [818CT12.1.1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:2000. IHC-P: 1:25. FACS: 1:25. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    818CT12.1.1
  • Conjugate
    Unconjugated
  • Gene ID2324
  • Target name
    FLT4
  • Target description
    fms related receptor tyrosine kinase 4
  • Target synonyms
    CHTD7, FLT-4, FLT41, LMPH1A, LMPHM1, PCL, VEGFR-3, VEGFR3, vascular endothelial growth factor receptor 3, Feline McDonough Sarcoma (FMS)-like tyrosine kinase 4, VEGF receptor-3, fms related tyrosine kinase 4, fms-like tyrosine kinase 4, primary congenital lymphedema, tyrosine-protein kinase receptor FLT4
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP35916
  • Protein Name
    Vascular endothelial growth factor receptor 3
  • Scientific Description
    This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. Mutations in this gene cause hereditary lymphedema type IA. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (A01276-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF Receptor 3 antigen affinity purified polyclonal antibody (Catalog # A01276-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF Receptor 3 at approximately 153KD. The expected band size for VEGF Receptor 3 is at 153KD.
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