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ProductsBack/VEGFA Mouse Monoclonal Antibody [Clone ID: OTI2F7]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGF (RC223789, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGF.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGF (RC223789, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGF.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VEGF (RC223789, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VEGF.

VEGFA Mouse Monoclonal Antibody [Clone ID: OTI2F7]

CF500288
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetVEGFA
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    VEGFA Mouse Monoclonal Antibody [Clone ID: OTI2F7]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2F7
  • Gene ID7422
  • Target name
    VEGFA
  • Target description
    vascular endothelial growth factor A
  • Target synonyms
    L-VEGF, MVCD1, VEGF, VPF, vascular endothelial growth factor A, long form, vascular endothelial growth factor A121, vascular endothelial growth factor A165, vascular permeability factor
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP15692
  • Protein Name
    Vascular endothelial growth factor A, long form
  • Scientific Description
    Carrier-free (BSA/glycerol-free) VEGF mouse monoclonal antibody, clone OTI2F7 (formerly 2F7)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VEGF using anti-VEGF antibody (PB9071). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysate, Lane 2: rat lver tissue lysate, Lane 3: rat thymus tissue lysate, Lane 4: mouse lung tissue lysate, Lane 5: mouse thymus tissue lysate, Lane 6: HEPA1-6 whole cell lysate, Lane 7: human placenta tissue lysate, Lane 8: A431 whole cell lysate, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF antigen affinity purified polyclonal antibody (Catalog # PB9071) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF at approximately 27KD. The expected band size for VEGF is at 27KD.
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