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Western Blot analysis of Jurkat cells using VEGF-B Polyclonal Antibody
Western Blot analysis of Jurkat cells using VEGF-B Polyclonal Antibody
Western Blot analysis of Jurkat cells using VEGF-B Polyclonal Antibody

VEGFB Antibody

CSB-PA004444
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetVEGFB
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Overview

  • Supplier
    Cusabio
  • Product Name
    VEGFB Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7423
  • Target name
    VEGFB
  • Target description
    vascular endothelial growth factor B
  • Target synonyms
    VEGFL, VRF, vascular endothelial growth factor B, VEGF-related factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49765
  • Protein Name
    Vascular endothelial growth factor B
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of VEGFB using anti-VEGFB antibody (A04494-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat pancreas tissue lysates, Lane 3: mouse cardiac muscle tissue lysates, Lane 4: mouse skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGFB antigen affinity purified polyclonal antibody (Catalog # A04494-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGFB at approximately 29KD. The expected band size for VEGFB is at 22KD.
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