WB analysis of various samples using GTX00863 Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01]. Arrow shows subunit A (32kDa) of VT1 and VT2. Lane 1 : Culture medium of E. coli O157:H7 Lane 2 : Purified VT1 Lane 3 : Purified VT2 Lane 4 : VT non-producing E. coli
![ELISA analysis of crude extract of E.coli O157:H7 using GTX00863 Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01]. Protein coating amount : 100microl (1 microg/ml) per well Antibody amount : 100microl at indicated dilution](https://www.genetex.com/upload/website/prouct_img/normal/GTX00863/GTX00863_20200406_AP_009_18_w_23053121_265.webp "ELISA analysis of crude extract of E.coli O157:H7 using GTX00863 Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01]. Protein coating amount : 100microl (1 microg/ml) per well Antibody amount : 100microl at indicated dilution")
![Reactivity of GTX00863 E. coli SLT-1 + SLT-2 antibody [vero-01] with various food poisoning bacteria in immunochromatographic strip test. GTX64191 Vero Toxin1 (E. coli) / Shiga Toxin (S. dysenteriae) antibody was coated onto a specific area (test line) of a nitrocellulose membrane, while goat anti-mouse IgG was coated onto another specific area (control line) on the same membrane. Extract of each strain of food poisoning bacteria was mixed with the monoclonal antibody conjugated with colloidal gold. The strip was soaked in the mixture and reacted. (1) E. coli O26, (2) E. coli O111, (3) E. coli O157:H7, (4) E. coli K12, (5) Salmonella Enteritidis, (6) Campylobacter jejuni, (7) Staphylococcus aureus, (8) PBS. MAb (vero-1) reacted with EHEC strains, O26, O111 and O157:H7, but not with other food poisoning bacteria.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00863/GTX00863_20200406_AP_050_19_w_23053121_284.webp "Reactivity of GTX00863 E. coli SLT-1 + SLT-2 antibody [vero-01] with various food poisoning bacteria in immunochromatographic strip test. GTX64191 Vero Toxin1 (E. coli) / Shiga Toxin (S. dysenteriae) antibody was coated onto a specific area (test line) of a nitrocellulose membrane, while goat anti-mouse IgG was coated onto another specific area (control line) on the same membrane. Extract of each strain of food poisoning bacteria was mixed with the monoclonal antibody conjugated with colloidal gold. The strip was soaked in the mixture and reacted. (1) E. coli O26, (2) E. coli O111, (3) E. coli O157:H7, (4) E. coli K12, (5) Salmonella Enteritidis, (6) Campylobacter jejuni, (7) Staphylococcus aureus, (8) PBS. MAb (vero-1) reacted with EHEC strains, O26, O111 and O157:H7, but not with other food poisoning bacteria.")
WB analysis of various samples using GTX00863 Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01]. Arrow shows subunit A (32kDa) of VT1 and VT2. Lane 1 : Culture medium of E. coli O157:H7 Lane 2 : Purified VT1 Lane 3 : Purified VT2 Lane 4 : VT non-producing E. coli
Verotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01]
GTX00863
ApplicationsWestern Blot, ELISA, Other Application
Product group Antibodies
ReactivityBacteria
TargetstxA2
Overview
- SupplierGeneTex
- Product NameVerotoxin / Shiga toxin (SLT-1 + SLT-2) subunit A antibody [vero-01]
- Delivery Days Customer9
- Antibody SpecificityIt reacts with subunit A of VT1, VT2 and Stx.
- Application Supplier NoteWB: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDvero-01
- Concentration1 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID1261950
- Target namestxA2
- Target descriptionShiga toxin Stx2c subunit A
- Target synonymsL0103; Shiga toxin Stx2c subunit A; sltIIA; ST933Wp40; stx2A
- HostMouse
- IsotypeIgG1
- Protein IDP08026
- Protein NameShiga-like toxin 1 subunit A
- ReactivityBacteria
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203